EB virus nucleic acid detectio ±n kit (PCR-fluorescent probe me↑σ♣&thod)

Clinical background

Epstein-Barr virus (EBV) is a member of↓↕☆ the genus Lymphotropic Virus of the Herαε§₽pesviridae family, and its genome is DNA.'¥® Epstein-Barr virus has the biolo★φgical characteristics of s$↓pecifically infecting humans and certain primatσ≤e B cells in vivo and in vitr♠×o. Humans are hosts of Epstein-Bar₩‍₩r virus infection, spread mainly ™♣← through saliva. 90% of adults worldwide are p×$×ositive for EBV antibodies. The fir≈✔φst infections in Asia and deve$€>&loping countries often occur in•× children, and the f≠↑irst infections in the United States and Westernσ&•λ Europe often occur in adults. Diseases relate✔÷•d to Epstein-Barr virus include i₹♦β↑nfectious mononucleosi™γ's, hemophagocytic syndrome, chronic active Epste±∞♣in-Barr virus infection, X-linked lymphopro ₹liferative disease, etc. I↔≤αεn particular, it is worth noting t>≠©±hat EB virus is an important tumor-related vir∑±λ us, which is closely related to the occur₩↓¥rence of various tumors such as nasopharyngeal ✘÷∑↑cancer, lymphoma, and gastric cancer. The nu÷↓mber of people affecte≠‌✔‌d by EB virus-related tumors in the world reache₹∑s 1%.
Studies have shown that the posi'≠✔tive rate of EBV VCA-Ig∏₽G antibody in children aged 3 to 5 years in Ch∑→ε↓ina is more than 90%, and most children have☆" no obvious symptoms after→λλ infection, or cause mild pharyngitis and upper™★ respiratory tract infection. Primary infectio <'↕ns occur in adolescence, anσ♦d about 50% of them develop infectious m↓≈"ononucleosis. It is mainly spread through s₩Ωaliva and can also be transmitted t≠↔hrough blood transfusions.¥‍

Features

Based on real-time fluorescence PCR technolog∑λy, rapid detection, accurate and reliable resultsα↓.
The reaction system of this kit contains a dU-UN☆≈±G enzyme anti-pollution system to avoid false pos ∞itive results; the system contains in∑&↔≤ternal standards to avoid ↕₽®false negative results.
The highly conserved region of the EB vi₽ £≥rus genome is the target region,≈₹π₽ and specific primers a☆☆nd fluorescent probe≈£&εs are designed to perform PCR amplification÷φ← to ensure the accura₩∏‌cy of the product.
Carry out big data clinical verificatioδ≈n covering national represe×Ωntative clinical units, with 'outstanding product perf≤←‍∏ormance.

Product performance

EB virus nucleic acid detection kit (PCR-f☆♣<λluorescent probe method)
Sensitivity	5.0x103copies/mL
Linear range	1.0x104copies/mL ~ 1.0x≤✔÷✘ 109copies/mL
Accuracy	With reference to international reference >≈↔βproducts, the coincidence rate of the t♣'est results is 100%
Precision	Coefficient of variation within and between ₹π↔¥batches CV≤5%
Specificity	100% specificity, no cross reactio≤≠&n with Streptococcus pneumoniae, Mycobacterium tuberculosis, Myc‍₽↑oplasma pneumoniae, Pertussis baσ¶Ω cteria, adenovirus, respiratory sα≠∑yncytial virus, etc.
Anti-interference	Mucin, pus, erythrom™§ycin, chloramphenicol∞£♠‍ did not interfere with th<↔λe test results

Sensitivity and linear range

Figure 1: Using 1x1×&±04copies / mL refere≤∏¥nce as a template, repeaλ&♥t the 20-well test in the same batch, c♥↓alculate the coefficient of vari←©ation of the CT value≥♣€ in the batch, and the CV value is ≤5%,¶☆× with good consisten☆∏‍cy. product information
Figure 2: For the reference products i×₩☆n the concentration rang ✔e of 107-104 copies / mL, the prod§☆£λuct amplification curve is smooth and complete, ♣≤÷and the correlation coefficient betwe₹αen different gradients is R> 99%, and tΩ∞he correlation is good.

Application field

For diagnosis of EBV infection-related£↔λ diseases in children
For the auxiliary diagno™δβsis of malignant tumors

product information

Test specimen : Whole blood sample
Technical principle : PCR-fluorescent probe method
Packaging specification : 25 tests/ kit
Category : In vitro diagnostic reagent
Applicable instrument <±©γ: Fluorescent quantitative PCR instrumentβ

Product Category