
Mycoplasma pneumoni→§ae (M. Pneumonia, MP) is the pathogen of human myπ↓coplasma pneumonia. Theδ☆ pathological changes of mycoplasm≈πa pneumonia are mainl×δ'÷y interstitial pneumonia, sometimes complicat¥♥♠₹ed by bronchial pneumonia, which is called≠♣×∏ primary atypical pneumonia. Worldwide, $→&™multiple studies have shown t←®₹hat the annual MP infection rate is ♣¥§9.6% to 66.7%, the infection rat<§e during non-epidemic period is 10×λπ% to 20%, and MP acc±★ounts for more than 30% of pedλ÷✘×iatric pneumonia during epidemic per₽γ$iod. There are approximately 156 Ωγ×million children with pneumonia each year, ♥<←of which 151 million children ♠©with pneumonia occur in ≤σ♦developing countries and are the leading cau← ×∏se of death for childr≤λ↓♥en under 5 years of age, accouα♠↕βnting for 19% of the total num↔¥ber of deaths. It is also the m<ain cause of death among adults, especially aπ$mong the elderly.
Mainly infected by droplets, the incubation perioγ≤∞d is 2 to 3 weeks, and the incidence is the highΩ↕est among adolescents. The clinical sy ♠£mptoms are mild, or e←§ven asymptomatic at all, and if t•π$÷here are only general respiratory •symptoms such as headache, sore throat, f♠ α↕ever, cough, etc., but there ha•¶♦ve been individual dea≠€'th reports. It can happen all year round, but £↓mostly in autumn and winte∑↓✔r.
Mycoplasma pneumoniae nucleic acid detection βσ✘ kit (PCR-fluorescent probe method) |
|
Sensitivity |
5.0x103copies / mL |
Linear range |
1.0x104copies / mL ~ 1.0x 109copies / mL |
Accuracy |
With reference to intern >ational reference products, the coincidenδ≈ce rate of the test results is 100% |
Precision |
Coefficient of variation within and betw♣β±¥een batches CV≤5% |
Specificity |
100% specificity, no cross reaction with Strep♣≈§∑tococcus pneumoniae, M∞¶ycobacterium tuberculosis, Epstein-Barr virus, peσ✔rtussis virus, adenovπ 'irus, respiratory syncytial virus, etc✘×™<. |
Anti-interference |
Mucin, blood, pus, erythromycin, chloramphen®£βicol did not interfere with the test results |
Figure 1: Using 1x104c₹πopies / mL reference as a template©↔γ≥, repeat the 20-well test in σ$ ←the same batch, calculate the coefficient"÷ of variation of the CT value ¶→÷in the batch, and the CV value is ≤5%, with go ↔€od consistency.
Figure 2: For the reference products≈÷≤↑ in the concentration range of 107-104£₽ copies / mL, the prodλ↑uct amplification curve is smooth and complete♣ €, and the correlatio±βεn coefficient between different gradie <nts is R> 99%, and t±" he correlation is good.
Date:2020-03-26
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