Adenovirus (ADV) is a group of ver™±y widely distributed viruses wi'∞<₽th double helix DNA genomes.€☆ Acute infectious diseases caused by ADV &βcan easily invade the mucous membra★∑nes of the respiratory tract and digestive tr↔←←act, ocular conjunctiva, urinary tract a €±✔nd lymph nodes, mainly manifested as "₽acute upper respiratory tract. Infection (<&2% to 4% of acute res¶βpiratory infections caused by'®↕" adenovirus), followed by ey ₹e and gastrointestinal iα'nfections. The population is gener€ ♠↔ally susceptible, more common in c δhildren, about half of the pa♦✔∑λtients are hidden infections. Infants and young♣ children are susceptible to adenovirus pneum∑≤onia, which accounts for₹"★' about 10% of childhood pneumonia. The co↕§↔ndition is serious and the mortality is high. ≠ The mortality of adenovirus pneumonia in young pφ£₹eople is 8% to 10%, and ther¶↑e is no specific treatment. According'δ to the investigation of serum specific antiε↔body and virus isolation, it can be seen↑♥ that adenovirus infection is←™$γ very extensive.
Figure 1: Precision (using 1.0x104PFU / mLα$₹ reference material as a template, repeate§♥d 20 times in the same batch to detect CV valγ≠↔&ue ≤5%)
Figure 2: Linear range (for 103 ~ 108PFU / mLφ✘ concentration range refer♠←♦ence products, the product a↕λ¶mplification curve is smooth and complete, the coγ↔×rrelation coefficient between dif≠ ferent gradients is R> 99%, and t≤α↕he correlation is good)
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Adenovirus nucleic acid detection kit (PC↔↓R-fluorescent probe method)♦α¥↔ |
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Sensitivity |
1.0x103PFU/mL |
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Linear range |
1.0x103PFU/mL ~ 1.0x 108PF£σ✔U/mL |
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Accuracy |
The positive coincidence rate was 100%, the ∑★negative coincidence rate was 9$€↕γ8.83%, and the overall coinε∞Ωcidence rate was 99.★γ41% |
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Precision |
Coefficient of variation within and between batcε☆hes CV≤5% |
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Specificity |
100% specificity, no cross re¶✘action with other respiratory pathogens |
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Anti-interference |
Mucin, blood, pus, erythro±©γ✔mycin, chloramphenicol did not ↔<→interfere with the test results |
Date:2020-03-26
Date:2020-03-30
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Date:2020-03-03
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