Product introduction
This product is a mixture of azure pigment, eosi↑₹n and methylene blue. T☆he chemical properties of various cell →δ→∞components are different, and the affinity forβ↕ various dyes is also di≠βfferent. Eosinophilic part≈≥>≠icles are basic proteinsσ☆, which are combined with eosi®≈≈↓n and dyed pink; eosinophilic particles, suεch as nucleoprotein or lymphocyte cytγ₽βπoplasm, are acid substances, which ×σ₽are combined with basic '♥dye methylene blue or azure and dyed purplσ≈σλe blue; neutral particles are in isoelectricσε state and can be combined with eosin and meth<≈ylene blue, which are light purple. All kinds of ™∞ components are distinπ guished because of their own characteristics and π₹the combination of diff∏×± erent substances in jimsa dye solution t♣o present different colors.
The Giemsa stain solution prepared by our ★¶★>company is made of imported Giemsa sta ♠Ω<in as raw material, which can dye the nucleus t≥ ✘o purplish red or blue purple, and the cytoplasmφ< to pink, presenting a clear cel$ $÷l chromosome image u≈↔×nder the light microscope. It is ↓ ☆mainly used to show the difference of the shape£× and size of various blood cells in bl☆↔ood smear, with good staining effect, strong st&λ"↑aining power and clear β≠ staining.
Dyeing effect
Hemoglobin and eosinophils were dyed pink; nucleu&•↓↑s, lymphocyte and basophil cyε±®toplasm were dyed purple blue α&or blue; protoerythrocyt≥≠es, early erythrocytes and nucleoli were✘σ dyed thick blue; middle and young erythrocytes w®↓ere dyed red blue or gray red; mature erythrocyte"₩→s were dyed pink.
Inspection principle±Ω↕$
The chemical properties of va ↓™rious cell components ar<÷♦e different, and the affinity for various dyes ↑is also different. Eosinophil♠ •₩s are basic proteins, which are com≥₩₹bined with eosin and dyed pink; nucleopr>↕∞∏otein and lymphocyte cytoplas ™<m are acid, which are combined with basic dy↕Ωe methylene blue or azure and dyed pur•♦ple blue; neutral particles of neε$ "utral substances are in isoelectric state, ↕which can be combined with eosin and methyl§σ±ene blue and dyed light purple.
[Product advantages] Stable dyeing ef☆₩→fect and fast coloring
[Storage conditions] Room temper¶ature and dark storage
[Shelf life] Two years
[Specification] 5ml, 50ml, 15±×≥♣0ml, 250ml, 500ml
Dyeing procedure (for reference only)
This product is the original so↑δ¥→lution. When using, take 1ml₩✘♦ of jimsa concentrated solution and 10ml of phosp×&horic acid buffer solution to mix well, an∏σd then it can be used.
Step 1. Prepare the split phas★✘e sections according to£≠£ the conventional method, dry them, dφ≈<δrop the diluted dye solution to cover all"♦ sections, and dye them at rδ₩•βoom temperature for 2-6 minλ↕ ♠utes.
Step2. Wash the slid ♠γe slowly from one end with tap water (pλ"ay attention not to wash™™ the slice directly), and conduct microsco>®pic inspection after drying.
Karyotype analysis and test method of periph£₩eral blood (for reference only)
1. Seed blood:
Under aseptic condition, 0.3-0.≈α↕5ml heparin anticoagulγπ♠ant whole blood was inoculated vertically i♣≈×γnto lymphocyte culture medium with→©"∏ No.7 needle.
2. Culture:
The cells were cultured in 37 ℃ con©₽stant temperature incubator for 68-72 hours, aΩ±nd the flask was shak®εen once every 24 hours'↓₹ to make the cells fully cult↓×'←ured.
3. Cytological treatme±ε₽nt:
3.1 add autumn: add 3-4 drops (about 30 μ L"•<♦) of colchicine with the co★δ☆ncentration of 40 μ g / ml to t €♥₩he 7-needle 2-3 hours before the comp×↔letion of culture, and then put it back "∑≠€into the constant temperatε'ure incubator at 37 ≠™α℃ for 2-3 hours.
3.2 sample collection: after t₩♣he completion of culture, pour tγ'he culture medium into tδ™ he corresponding number of 15 ml centrifuge t♣♥ube, 2000 R / min, centrifuge for 1®0 minutes, and remove the superna★ ↓tant.
3.3 low permeability: add 9ml of low permeabi≥γ lity liquid (KCl) with 37 ℃ ÷> water bath in advance to each tube. Blow↑"♦₽ 3 times for each tube,©&★ then 7 times for each tube, 28-30 minutes fo₽♥r water bath, and 10 times for each tube in λ✔∏the middle of 14-15 minutes. Formuδ'✘αla of hypotonic solution: weigh 5.6g K∞ΩγCl, dissolve it with 1000ml purified w★ ↔↔ater, and take a 37 ℃ wate>₹r bath before use.
3.4 pre fixation: add 1m©&$l of the fixed solution₹φβ¥ with 37 ℃ water bath ahead of schedule into e♦©∑ach tube completed by hypπ≈otonic treatment, blow three times in each• tube, and then blow sev↔φen times again. After 10 m←®inutes of water bath, centrifuge for 10 minutes a₹≈Ω¶t 2000r / min to rem✘ove the supernatant. Formula o∑®f fixed solution: mix ✔★ anhydrous methanol and glacia☆•♠÷l acetic acid according to "≤↔the volume of 3:1. It is now pr♦∏epared for use. Use 37 ℃ water bath before use.↑✔
3.5 fixation: add 9ml of f♦•ixing liquid into each tube, blow three timγ<¶φes first, and then blow seven times, Ω↕water bath at 37 ℃ for 10 minutes, centrif♣ ugation at 2000r / min for 8 minutes☆₽♣, and remove the supernatant. Repeat this ♣✔procedure two more times.
three point six Drop: (1) add 0.5ml ★☆ ±of fixed solution to each tube if there iσγs more precipitation, and do noγ t add fixed solution if there is ☆€less precipitation. After blowing ge ₩€αntly for 5 times, leave it at room tempera♣λture for 30 minutes, and the♣σn gently blow it for ≈₽5 times before dropping and mix it; (2) take out©±♦↑ the micro frozen glass slide,σ≥ mark it, drop 3-4 drops of sample, and♣δφ✘ bake it for 1 minute at 60 ℃; 3¥™) put the drop into 90 ℃ electri•♦c blast drying oven 1 5 hours.
4. Chromosome banding:
4.1 pancreatin digestion: put the processed λ∞♦slides into 0.025% pancreatin solutio> n (ph7.2-7.4) which is ←"™✘preheated to 37 ℃ for dige φ∞stion for 4 minutes and 20 seco₽Ωnds, and then rinse them in 0.9% NaCl solut♦δ≠ion which is preheated©φδ± to 37 ℃ for 3 times.
4.2 dyeing: dye in Giemsa solution t&☆hat is preheated to 37 ℃ for 3 min↓$≠utes, wash with tap water, dry or dry, and$ then read the tablet.
