Glucose-6-phosphate dehydrogenase(G6PD×₩) deficiency is one of the most common >λ×X-chain incomplete genetic diseases in the w£γorld, commonly known as broad bean diseas× e. Clinically, it is manifested as neonatal jaund↕ice, broad bean disease, drug-based hemo★∑÷lytic, infectious hemolytic and non-sphericΩ©® al cell hemolytic anemia and other diseas§Ωes. In children and §γ₩ infants concentrated in ↓"the performance of broa£π♣d bean disease and neonatal πjaundice, often more serious, if not timel★πy treatment will endanger the lives o♣←f children. About 200 million people™∞× worldwide are affected. About ×₽50% of newborns with G6PD defi< ciency develop neonatal jaund≤¥ice, and about 12% ™π©;of them develop nuclear jaundice, ₩π©leading to brain damage and low intelligence.
|
1376N |
1388N● |
487N● |
95N● |
392N● |
871N● |
592N ● |
|
1376M● |
1388M |
487M |
95M |
392M |
871M |
1004M● |
|
1381M |
1387M |
493M |
592M |
1004M |
1024M |
1024M● |
A: Genetic testing, the ¥±results are accurate and reliable for life.
B: For patients with G6PD gene mutation, clea→βγr and targeted medic ↕∑ation guidance can be given to∏βφ avoid contact with dr↕$βugs that may cause acute hemolysis.
C: Genetic testing can effectively detect fe≠×male heterozygotes and neonatal hemolysis✘&™•.
D: Definite family gen☆♦✘etic history. Provide guidan∑ ✔ce on marriage and childb∑≠irth and antibiotic medica✘tion.
E: Early detection of G6PDλβ deficiency caused by genetic factor≈£↔×s. Newborns can start pre≠€★'ventive interventions from their motε¶αhers.
|
Comparison items |
Chemical methods |
Melting curve method |
Glass chip method |
PCR Reverse Point Hybridization (Billion Cube) |
|
Detection accuracy |
Unable to detect female&nb"π♦★sp; hybrids, affected by environmental impact |
Small differences in •© mutation temperature of adjacent sites can lead to miscalculation of results |
Chip hybrid conditions are difficult to unify,&n εbsp; and specificity needs to be improved |
More than 99.9% of conformity with sequencing&nbsα®♦∑p; control results |
|
Detecting sites |
No-type |
12 sites |
7 sites (including 1♦€ non-pathogenic site) |
12 sites |
|
Instrument |
Biochemical instrument |
Fluorescence quantitative PCR instrument |
Requires dedicated equipment |
Common hybrids |
G6PD deficiency genotyping det©★✔ection
Risk assessment of G6PD deficiency in child care δ"before marriage examination
Prenatal G6PD deficiency screening hig≥ ≠h-risk groups, newborn screening
G6PD gene mutation population to check allergies €δbefore using antibiotics
Test specimen:Anticoagulant whole blood sγ"ample
Technical principle:P₽←CR - reverse point hybri<δdization
Packing size:25 tests / kiε☆t
Class:In vitro diagnostic r±₽eagents
Suitable instruments:Common gene amplλ®•ification instrument, molecular hybri €≤dizer
Date:2020-03-26
Date:2020-03-30
Date:2020-03-17
Date:2020-03-11
Date:2020-03-03
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