Parainfluenza virus (type I, II"φ÷γ, III) nucleic acid detection ≠ •♦kit (PCR-fluorescent probe method)
【Clinical manifestations∞ ✔】
Parainfluenza viruses can mainly cause low↔±↕ er respiratory tract infections in children, a₹λλnd its pathogenicity is second onl©≈γ↕y to respiratory syncytial virus (RSγ₩$V) . Human parainfluenza virus can cause upper☆¶ respiratory tract infections such as recurre•☆λ↔nt colds and sore throats. It can also cause seve¶γre recurrent infections of pneum↑←✔onia, bronchitis, and bronchiolitis of lower resp↕÷↕¶iratory tract diseases. The lower respirator✘✔↔y tract infections caused ma£↓εinly occur in the elderly and ><© in people with immunodeficienc®≈y.
【Features】
This kit uses real-time fluorescent PCR te$★&chnology, with highly conserved regions of th→ ↑e parainfluenza virus ( typ&®"es I , II , and III ) a→♣≈s target regions, designing specific primers and →←fluorescent probes, and then performε£↔£ing PCR amplification on ✔✘∞the target sequence , while using Ta≈♠>qman Fluorescent probe technology mo≤>&♥nitors the accumulation of amplified products i✘αn real time to achieve qualitative detection o×λf respiratory syncytial virus nucleic aci±'ds in samples . The reaction system of ≥this kit contains dU-U§ δNG enzyme anti-pollution system to §®<avoid false positive results.
【Product Performance】
|
Sensitivity
|
1.0x10 3 copies / mL
|
|
Linear range
|
1.0x10 3 copies / mL ~ 1.0x 10 9 copies / mL
|
|
Accuracy
|
The detection result coincidenceΩ rate is 100%
|
|
Precision
|
Coefficient of variation within π&and between batches CV≤5%
|
|
Specificity
|
100% specificity , no cross reaction with influenza A virus, influenza B virus,ε≤ adenovirus , human Boca virus, Epstein-Barr &₹★virus, Mycoplasma pneumoniae, Chlamyd✘λ¶ia pneumoniae, etc.
|
|
Anti-interference
|
Oxymetazoline hydrochloride§÷↓★ spray, budesonide nasal spraγ↓₩☆y , zanamivir , riba↕'α$virin did not interfere≤∞ with the test results
|
【Product information】
Test specimen: throat swab
Technical principle: PCR- f♠¶≠↑luorescent probe method
Packaging specifications: 24 tests/ kit, 48 ÷¶←tests/ kit, 96 tests/ kit
Applicable instruments: 'π Roche Cobas z 480 ; Roche Lig♥☆✔ htCycler 480 ; ABI 7500 ;Ω≠ Shanghai Hongshi SLAN-96S ; Bioer ↕LineGene 9600, etc.
