Norovirus nucleic acid detection kit (PCR-fluorescent probe method)
Norovirus nucleic acid detection kit (PCR-fluorescent probe method)

Norovirus nucleic acid detection kit (PCR-fluore÷≤‍scent probe method)

【Clinical manifestations】

  Norovirus  is considered to be ∏£®the major cause of non-bacterial pathog₹≠€↔en outbreaks of acute gastroenteritis, often∏  causing vomiting, diarrhea and oth∏δπer symptoms, can be transmitted th​₹rough a variety of wa±≠β‌ys food, water and so on. Norovirus viru<☆s divided . 5 genome, wherein​¶ G Ⅰ, G Ⅱ Norovirus is the most co™'> mmon worldwide genome, reported to hav©←e a global epidemic diffusion. Norovirus i'☆≥☆s mainly infected through the fe™ $cal-oral route. After infection ↓, humans and animals can ≥π secrete a large number of no​ ©✔rovirus particles. Innovirus has strong ‍<εviability and infectivity in vitro. Norovirusσδφ can be infected in small ←©≤φdoses (<100 virus parΩ©ticles), and the population is generall₽φ∑'y susceptible to it. The clinica$λ→Ωl symptoms of the human body after being infe ♦•cted with Norovirus ar÷ε₩e mainly vomiting and diarrhea, an♣★☆βd it is difficult to distinguish the sympσ £toms of gastroenteritis caused by other pathogensδ&.

【Features】
  This kit utilizes real-time fluorε≤♣escence PCR technology to ★♣Ωnorovirus G Ⅰ, G Ⅱ type genomic reg>→₹€ion is highly conserved tar∏✘↓get region, the design o™✔♥₽f specific primers and fluorescent±≠♥γ probes, and target sequences for PCR a>♦✔≤mplification, through Taqman fluoresce♦♥Ωnt probes Needle technoloε∏♥↓gy monitors the accumulation of amplification pr₽∞oducts in real time, enabling qualitative dγ↓↓etection of Norovirus nucleic acids in samples . ✔'≤£The reaction system of this kit cont₹✔¥€ains dU-UNG enzyme anti-polluti♦‌↓•on system to avoid false positive results .
【Product Performance】

Sensitivity

1.0x103copies/mL

Linear range

1.0x103copies/mL ~ 1.0x 109copies/mL

Accuracy

The detection result coincide ≥σnce rate is 100%

Precision

Coefficient of variation within a↓Ω₹nd between batches CV≤5%

Specificity

A specificity of 100% , with ₹±​the wheel-like virus, S★γ∑apporo virus, 

enteric adenovirus virus, stellate virus, Salmon✘" ella, 

Shigella and other non-cross-reactive

Anti-interference

10% pus blood, 0.5% erythromycin suppos♦§itory, 

0.5% anal Thai suppositorγβ€y, etc. did not significantlπ§y interfere with the test results

【product information】
Test specimens: feces, anal swabs
Technical principle: PCR- fluorescent probe method
Packaging specifications: 24 tests/ kit, 48 tests/ kit, 96 tests/ kit
Applicable instruments: Roche Cobas z 480 ; Roche LightCycler δ✔₹>480 ; ABI 7500 ; Shanghai Hongs↓™hi SLAN-96S ; Bioer LineGene 9600, etc.