Boca virus nucleic acid detection kit (PCR-flu ↕αδorescent probe method)
【Clinical manifestations】
Bocavirus is a genus of∑¥£↑ the Parvoviridae subfamily of the Parvov↔↓iridae, first discovered in a•πnimals in the early 1960s. In ↔÷♥"August 2005, Swedish scienti©φ≈πsts used molecular virus screening methods fo">π←r the first time to discover a new ¶≈type of human parvovirus in children's respir±γφ∏atory secretions, named human Boca virus, ©₹which is closely related to ε human acute respiratory infections, •★γφand the symptoms are common Colds arπ →φe similar. The same virus was found in Au ↑φstralia and China, and the infection rat←&↑e was greater than 5.6%.
【Features】
This kit uses real-time fluorescent'← PCR technology, takes the highly conserveλ<∑d region of the Boca virus genome as the tar♠♥←≤get region, designs specific pri ↑mers and fluorescent probes, and tγ&hen performs PCR amplification on the target se÷≥∑→quence, while monitoring the amplificat♣×®₩ion in real time by Taqman fluorescent ♥< ×probe technology The accumulation of ★←products enables qualitative •♦detection of Norovirus nuclei&>Ωεc acids in the sample.↑ The reaction system₽☆ of this kit contains≈✔ ✘ dU-UNG enzyme anti-pollution system to¶₩> avoid false positive results.
【Product Performance】
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Sensitivity
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1.0x103 copies / mL
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Linear range
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1.0x103 copies / mL ~ 1.0x 109 copies / mL
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Accuracy
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The detection result coinci≥←€dence rate is 100%
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Precision
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Coefficient of variation wi¥φthin and between batches CV≤5%
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Specificity
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100% specificity, no cross reaction&n€♣bsp;with influenza A virus, influenza B vi $rus, adenovirus , human metapneumo≈γσλvirus, rhinovirus, Epstein-Barr virus®↑∏¥, Mycoplasma pneumoni±•ae, Chlamydia pneumoniae, etc.
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Anti-interference
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Oxymetazoline hydrochlorideφ< sprays, budesonide nas£al spray, azithromycin neomycin,&nbs$∞p;zanamivir, ribavirin ≠±;and the like without significant interfere↕α§÷nce to the detection result
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【Product information】
Test specimen: throat swab
Technical principle: PCR- π fluorescent probe method
Packaging specifications: ÷≠24 tests/ kit, 48 tests/ kit, 96 tests/ k π↑it
Applicable instruments: Roche Cobas z λ€480; Roche LightCycler 480; ABI 7500; Sα£hanghai Hongshi SLAN≤♣™-96S; Bioer LineGene 9600, etc.
