L. pneumophila nucleic acid detection kit (PCR-fluorescent probe method)
L. pneumophila nucleic acid detection kit (PCR-fluorescent probe method)

L. pneumophila nucleic ♦✔∑acid detection kit (PCR-flu ↕>orescent probe method)

【Clinical manifestations】
  Approximately 90% of Legionellosis is‌α"' caused by Legionella pneumophila. The causatiβ>®ve agent of Legionella÷☆€♣ pneumonia is Legionella pneumoph↑βila, which accounts for 2 to 5% of communityφ÷-acquired pneumonia . The symptoms of Legionella ∏≥σdisease vary widely, from common cold and low™Ω fever to high fever,≠  with changes in mental status and respira∞•tory failure; atypical symptoms usually occu ☆r early in the disease,‌§ including headache, musclφ♥e aches, anorexia, fatigue, etc. . Diarrhea and o≤÷ther gastrointestinal sympt↕‍oms are approximately 20-40% . Among the atypica•"♣✘l cases of community-acquired pnφδα✔eumonia, Legionella di♦¶←sease is the most serious clinically‌‍₹←, and if not treated properly and in ti§✔me, it will progressi<♥↕vely worsen. Although there are few ext ☆rapulmonary symptoms, it can also cause myocar♥ ditis, pericarditis and artificial valve‌÷≤ endocarditis, as well as glomerulonephritis, pan×>£Ωcreatitis and peritonitis.
【Features】
  This kit uses real-time ∑‌≥✔fluorescent PCR technology, takes the highly₹  conserved region of the Legionella pneumoph& σ‌ila genome as the tar±∑get region, designs specific primers anε©™d fluorescent probes, an​±d then performs PCR amplification on the target s¶‍equence , while monitoring real-tim​↕e expansion through Taqman fluorescent‌•  probe technology. Accumulati÷↕on of augmentation products enables"§♥© qualitative detection of Norovi∞λrus nucleic acids in sampl¶₽↓es . The reaction system of this kit₹♣↔ contains dU-UNG enzyme anti-pollut♦∑→ion system to avoid false positiv§₩×‌e results .
【Product Performance】

Sensitivity

1.0x103copies/mL

Linear range

1.0x103copies/mL ~ 1.0x 109copies/mL

Accuracy

The detection result ÷♠coincidence rate is 100%

Precision

Coefficient of variation within and be£π•♦tween batches CV≤5%

Specificity

100% specificity , no cross reactio¶‍βn with Staphylococcus aδΩβureus, 

respiratory syncytial vi$£rus, Epstein-Barr virus, Haemophilus influe¥≥≥≤nzae, 

Mycoplasma pneumoniae, Streptococcus pneumoniae, ≈δφ£etc.

Anti-interference

Oxymetazoline hydrochlorideγβε spray, budesonide nasal spray, er÷±Ω♥ythromycin, 

roxithromycin, azithromycin, e¶≠$‌tc. did not significantly interfere w✘φ×ith the test results

【Product information】
Test specimen: throat •ε♥swab
Technical principle: PC>֤R- fluorescent probe method
Packaging specifications: 24 tests/ ki✔π×t, 48 tests/ kit, 96 tests/ kit
Applicable instruments: Roche Cobas z 48 " 0 ; Roche LightCycler 480 ; ABI 7500 ; Shang×∞hai Hongshi SLAN-96S ; Bioer LineGene 9600, ☆₽•etc.