Molecular diagnosis and nu∑× $cleic acid detection have been widely us∞$ed in thediagnosis of infectious orε> genetic diseases, non-invasive ≠"→™prenatal detection,ep"≈♦®idemiological investigation, pathogφ≥enic microorganisms d≈↔etection and scientificresearch. Compared wi←th immunological detectio<¥n techniques, nucleic a§↓cid detectioncan obtain more ∞®↕αsensitive and accurate results. The×↔↔' first step in molecular diagnosiπ÷sinvolves the extraction of nucleic acids, which are usually bound to organic matter incells, such as his✔→®αtones. The extraction of nucleic acids r¥€λ↓equires first lysing the cells,and thenφ¥δδ separating the nucleic ac≈ ids from macromolecular ∑organic substances (su✔★chas proteins, polysaccharides, and lipids), w→hile the primary structure of the nucσ✘↓δleicacids must remain intact. The nucleic acid ex♶traction process currently depends onqualifi•✘≈ed professionals to o©ε$γperate. However, with the rapid development≥←← of nucleicacid detec×∑<tion technologies and clinical<≥ diagnosis, there is an urgent need for asimp≠§le and easy-to-use method suitable for on-&λ>site DNA extraction and detection.This met★♦₩hod needs to be widely applicable to technica≥≤l requirements for nuc÷™leic acidextraction from↕↕ primary hospitals, testing sites and eve×₩n the general public.
This product is based on silica ₹♣&Ωgel column purification. The sample is lys₹εed, and theDNA/RNA is">→ released into the lysis buffer. AΩ♠≈₩fter transferring to an adsorption columnand filtγ' er, DNA/RNA is adsorbed to th★$e membrane, while the una→ε✘♥bsorbed protein isremoved by filtrati±€<on with the solution. ®β→After washing away the prot≤↓ein and otherimpurities, DNA/RNA is fi ✔ nally eluted by a low-salt buffer to obtain a hig ☆h-purityproduct
Applicable specimens: cell-free/low-cell DNA/RN÷✔☆A samples such as clinical or humβ anbody fluids, serum, plasma, soaking fluid, su±→pernatants from tissue homogenate,→ cellculture superna♣ ∞tant.
Technical principle: silica gel column ∑δ≤purification.
Packing specification: 96 tests/kit, 200 tests∏δ☆/kit.
Storage conditions: 18-25℃ room temperature for 12 mon ♣¥ths.
HIGH RECOVERY RATE
silica gel column can recover nuclei♠£σc acid molecules at α¶₽the picogram level.
HIGH SENSITIVITY
Recovering viruses DNA/RNA↕επ as low as 10 copies.
HIGH QUALITY
Meeting various downstream applications ↕₽αβsuch as reverse transcription,qPCR,enzyme digesti♦on,blot hybridization,etc.
GOOD STABILITY
Stable solution system ensures cons≤♥£istent results every time.
WIDE APPLICABILITY
Suitable for extract®"ing viral DNA from serum, plasma, milk, ce£™&ll culture supernatant,and variou ∞☆s cell-free body fluid s×✘♥amples.
FAST
Extraction of multiple samples can be compl β™eted in less than 30 minutes.
Date:2020-03-26
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Date:2020-03-03
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