Respiratory↕✘γ> tract infection is one o★↑f the most common infectious diseases∏≤ in humans in the world, and the incidenc♣ ∞<e rate ranks first in various infectious d× γiseases. 20% of the deaths of♥&♠ preschool children worldwide are respirato φλry infections, causing 400 million people to ✘♦¶be infected with respiratory pathog∏<¥εens every year. The World Health ∑✔Organization (WHO) published an arti≠×cle in the "Lancet" magazine in 2005: i α♦n 2000-2003, 1060 per year Of the 10,000 ¶✘αchildren under the age of 5, deaths due to 'δγ✔infectious diseases accounted ₹for 54%, has become the main cause ♠₽Ω♥of death of children under the age of 5, and i₩§s also one of the public health'γ• problems of the elderly.
Based on PCR-fluorescent probe method,∏¥> qualitative detection of RNA and DNA of patho∞↕gens in pharyngeal swabs of subjects. α≤≈
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Project indicators |
7 immunofluorescence tests |
Single fluorescent PCR |
16 inspections ∞φof liquid chips (U.S. luminex) |
Capillary electrophoresis |
Method of this project |
|
Detection of pat∞♠π₹hogens |
7 inspections |
1 ~ 2 inspection☆γ₹ |
16 inspections |
16 inspections |
18 inspections |
|
Detection cycle¥♣£ |
2 hours |
3 hours |
6 hours |
4 hours |
1.5 hours |
|
Sensitivity |
low |
high |
high |
high |
high |
|
Sample flux |
1 |
6 ~ 12 |
96 |
48 |
48 |
|
Experimental operatio'€♦ n |
Extremely complex and slow ✔ • |
Relatively complex |
Relatively complex |
Relatively complex |
Simple and fast |
|
Product contamination ↑£× |
no |
Not easy to pollute ε |
Easily contaminated |
Relatively easy to pollute |
Not easy to pollute ®' |
|
Clinical charges αα |
low |
high |
high |
high |
low |
|
Respiratory pathogen multiple∏ §Ω typing detection kit (multiple ↓λ§πfluorescent probe melting c÷∞✔↑urve method) |
|
|
Pathogen type |
New coronavirus 2019-nCoV, ★ coronavirus (HCoV-229E, HCoV-OC43, HCoV-NL6↓±∑3, HKU1), influenza A♠γ↔÷ virus, influenza B virus,× respiratory syncytial virus, adenoviru©₩s, parainfluenza virus Type II, Type III), rhiφ∞novirus, human metapneumovirus, Bocaviru↓∞ ×s, B. pertussis, Chlamydia pneumoniae, M£ε ycoplasma pneumoniae←® . |
|
Sensitivity |
10 copies / μL ×ε |
|
Linear range π→♠¥ |
10-1.0x109 copies / μL |
|
Accuracy |
Use national reference •≠<products as the control, the coincidence ratγ↔>e of the test results is 100% |
|
Precision |
Coefficients of variation wit©πhin and between batches CV are le∏→♦ss than 4.5% and 3.5%, respectively β•≈ |
|
Specificity ₽₩™ |
100% specificity, no cr✘★oss-reactivity with ot¶♦<her clinically common pathogenic mi ♣croorganisms |
|
Anti-interference |
Commonly used drugs for the tr✘£eatment of respiratory disea¶Ω÷ses do not interfere with thi☆₩$s product |
Diagnosis of pathogen classificatio &n of respiratory tract pa£©±→tients
Respiratory tract risk ≤ assessment
High-risk groups, newborn scree≥>ning
Date:2020-03-26
Date:2020-03-30
Date:2020-03-17
Date:2020-03-11
Date:2020-03-03
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