Polymerase ch£§ λain reaction (PCR) is a molecular biol→σogy technique used to amplify and am∑✘¶♣plify specific DNA fra₩γ₹gments. High-efficiency amp£♣lification of selective fragments of genes$☆ in vitro can be used to deteΩ✔ct target genes.
The main principle of the PCR-fluor ✔escent probe method is: during PCR amplification$$¶™, a specific fluorescent probe±" € is added at the same time §βΩwhen a pair of primers are added, the pro•≠βbe is an oligonucleotide, and a γαreporter fluorescent group is labeled at both en'☆βds. And a quenching fluoro∞₽'phore. When the p&'robe is complete, the fluores®∏cent signal emitted byα¶✔ the reporter group is absorbed by the quenching ↕×group; with PCR amplifi✘✔cation, the 5 'end-3' end exonu♠>♦clease activity of the Taq e< ∏nzyme will degrade the probe λ↓to make the reporter fluorescent group The group •αand the quenching fluores $&πcent group are separated, so that t₹≠he fluorescence monitoring system ca×₹n receive the fluorescent↓£ signal, that is, every <φtime a DNA strand is amplified, a fluorescent mol ®ecule is formed, and the accumulation of the •✔ fluorescent signal and the PCR produc₽Ωt formation are comple'↑™tely synchronized.
Date:2020-03-26
Date:2020-03-30
Date:2020-03-17
Date:2020-03-11
Date:2020-03-03
WeChat official account