The main princip♣®&♠le of PCR fluorescent probe melting cur ✘☆₩ve technology is to design m₩₹olecular beacon probes for the♣≈γ♠ sites to be detected; after≠ PCR amplification is completed, the ♦ £♦amplified products are hybriφ≠♦≈dized with molecular beacon probes, then sloε ≠wly rise Temperature, d↓ε×o the melting curve analysis of th€× ™e probe and the product, and read the genetic pol÷★ymorphism information of the site accor£φε♦ding to the temperature correspondi®€★ng to the melting peak. Co<€β≈mpared with other methods, it has↕σ the advantages of simple operatio€ n, high accuracy, good specificity and easy to λ¶&∏judge results.
Date:2020-03-26
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