PCR-electrophoresis

  The main principle₹δ​∏ of the PCR-electrophoresis met✔πhod (Gap-PCR method) is to c≠±onduct electrophoresis of PCR products; a±™®☆nd the charge effect and molec♣δβ£ular sieve effect of±←≥δ DNA molecules while swimming. Un≠✘§✔der a certain electric field strength, the migra  tion speed of DNA moleculε es depends on the molecular sieve effe←®ct, that is, the size and configuration oβ©™₩f the molecules themselves. Therefore,<✘∏ different DNA molecules are isolat‍‍≥₩ed and compared with Marker to determine tσ↕∑¶he result. The PCR-reverse point hybrid®↓Ω♥ization method uses a biotin-label♣​€★ed probe to hybridize with a specific amplifie≤↑d PCR product, and a h≥φybridization signal ca↕₹★σn be displayed through a co✘©λ±rresponding color reacti★₹&on to judge the result.