PCR-reverse dot hybridization

  PCR-reverse ↕"dot hybridization is ↔≈ γcommonly used for genotyping and✔÷ gene mutation detection. ₹βThe main principle is to point✘​ the probes to be used to the nitrocelluloφ≤∑se membrane or the nylon membrane, each proδ✔Ωσbe is marked with a number,&n"®₹∑bsp; and then the DNA sa₽λ&mple to be tested (us✔‌®×ually the product spφ'ecifically amplified by PCR) , The bioti$§ ≥n label is pre-labeledφ≠Ω∑ on the 5 'end of the PCR γ¥™primer, so that the amplified product is correspo•↓ndingly labeled with biotin) to hyγ≈¶bridize with it, so that the sample to ↕ be tested will be combined with a probe h&δ↑★aving a homologous sequence, and unbound D•<"εNA is washed to remove In the sample,¶÷ since the DNA sample to be teste∑δd has biotin-based markers, t♣↓he probe points combined with th¥∑e DNA to be tested carry biotin-based m✘σarkers, and then the hybrδ©∞idization signal can be displayedσ←¶σ by the corresponding c✔★olor reaction.